|Title||Primer extension capture: targeted sequence retrieval from heavily degraded DNA sources.|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Briggs, AW, Good, JM, Green, RE, Krause, J, Maricic, T, Stenzel, U, Pääbo, S|
|Journal||J Vis Exp|
|Keywords||Ancient DNA, genomics, Neandertal DNA, sequencing, technology|
We present a method of targeted DNA sequence retrieval from DNA sources which are heavily degraded and contaminated with microbial DNA, as is typical of ancient bones. The method greatly reduces sample destruction and sequencing demands relative to direct PCR or shotgun sequencing approaches. We used this method to reconstruct the complete mitochondrial DNA (mtDNA) genomes of five Neandertals from across their geographic range. The mtDNA genetic diversity of the late Neandertals was approximately three times lower than that of contemporary modern humans. Together with analyses of mtDNA protein evolution, these data suggest that the long-term effective population size of Neandertals was smaller than that of modern humans and extant great apes.
|Alternate Journal||J Vis Exp|
Primer extension capture: targeted sequence retrieval from heavily degraded DNA sources.
For years, I've worked on their bones. Now I'm working on their genes. Read more about the science studying these ancient people.
From a finger bone of an ancient human came the record of a completely unexpected population. My lab is working on the science of the Denisova genome.
The advent of agriculture caused natural selection to speed up greatly in humans. We're uncovering some of the ways that populations have rapidly changed during the last 10,000 years.